Ligase

Ligase is an enzyme that catalyzes the formation of phosphodiester bonds between nucleic acid strands, joining adjacent DNA or RNA fragments during in vitro reactions. It is widely used in molecular cloning, next-generation sequencing library preparation, PCR product ligation, and nucleic acid amplification-based diagnostic assays. Key performance attributes include reproducible activity, low nuclease contamination, and stability under standard reaction conditions, supporting reliable downstream results. Typical users include biotechnology firms, pharmaceutical R&D laboratories, clinical diagnostic developers, and academic research institutions requiring dependable enzymatic reagents for analytical and preparative workflows.